Press E to activate this mode. When pressed, Ayano Aishi will become Ebola-chan. She will become deathly pale with golden eyes. Her hair is styled in waist-length pink pigtails, with a white flower in it. The tips of her pigtails have a curl that looks similar to the Ebola virus and glow red. Ayano will also have small light-purple bat wings and her uniform will be recolored to resemble a nurse's outfit. When idle, her arms will be splayed happily outwards in the “Girly” pose.
If you press V 10 times, you will change to Corona-Chan, a skin of Ebola mode that has the same effect and idle animation, but a different appearance. She has black hair with two corona-virus cells on top of it. She also has a red dress, which exposes her bust.
Any NPC with a complete AI she touches will instantly die (presumably from Ebola) and will exude a visible white vapor that resembles a skull. Teachers can be killed in this mode if they touch Ebola-chan.
- If Ayano tries to pick up or drag a corpse, after dropping it, she will no longer have a smile, nor the Ebola-chan animation.
- If Ayano takes off the school uniform, her skin will become regular instead of white, and her eyes will be the default black instead of gold.
- This mode was implemented in the May 4th, 2016 Build.
- If an NPC is killed by Ebola-chan, the NPC will have a yellow outline in Yandere Vision. However, teachers, Senpai, and Midori Gurin will have the regular orange outline.
- YandereDev publicly promised to implement this mode in 2014. It only took an hour for this mode to be implemented.
- Genka Kunahito, Kocho Shuyona, and Taro Yamada cannot be killed with this mode.
- The hair was modeled by Druelbozo.
- YandereDev joked that since some people were offended by this mode, he should have added Malaria-sama, AIDS-san, Cancer-san, and Bubonic Plague-sama.
- The music is shared with Tornado Mode, Blade Hair Mode, Gazer Mode, and Medusa Mode.
- The music used to be "Remnant Of Twilight" from the game Hyrule Warriors.