This mode is a reference to the Hitman series. Press L to activate this mode. It cannot be deactivated until the game is reset or the day ends. Once pressed, Ayano will resemble the main character Agent 47. 47 Mode will make her bald, put a bar code on the back of her head, and change her uniform to a black jacket with a red bow. The song, Ave Maria will play the entire time.
- If Ayano wears gloves while in 47 Mode, her uniform texture will break.
- This mode was implemented in the June 17th, 2015 Build.
- Press D to disable the copyrighted music.
- The game's warning referenced this mode, saying that this game contains "bald assassins". However, YandereDev removed the warning in the March 17th, 2016 Build because he thought it was "cringey."
- The player is able to become bald with the bar code by pressing the H button until they reach the final hairstyle.